Leica SP8 Inverted gSTED


Leica SP8 Inverted gSTED

       Overview:

  • Sensitivity: ★★★★★
  • Speed: ★★☆☆☆ (galvano), ★★★★☆ (resonant)
  • Detection channels: 2 HyD, 2 PMT, 1 Transmitted Light
  • Recommendation: Use if your sample requires high sensitivity, has non-standard excitation/emission parameters, and does not require high speed. Gating is also available if your sample has autofluorescence issues.

       Detailed Features:

  • Diode Laser 405nm
  • Pulsed White Light Laser ranging from 470-670nm: Useful for live imaging, less harsh on samples. Gating function available to isolate small molecule fluorescence from protein fluorescence
  • Argon Laser: 458, 476, 488, 496, 514nm
  • STED laser: 592nm for Super-Resolution Imaging of up to 40nm
  • Multidimensional imaging (XYZT)
  • High Precision Galvo Z stage for Automated Imaging and stitching of large area of datasets
  • Simultaneous Spectral Imaging
  • Hardware Autofocus
  • 2 Hybrid Detectors (HyD) for sensitive imaging, 2 PMT detectors and 1 Transmitted Light Detector
  • Resonant Scanner: Fast Live Imaging of up to 2k x 2k
  • LASX Software equipped with Navigator; Focus Mapping and Lightning (A patented Deconvolution software)
  • Other Imaging techniques: FRAP, FRET, Photostimulation, and Spectral Imaging
  • Excellent for Live imaging, Subcellular Protein or gene Localisation and super-resolution imaging

Location: #02-27

Download the instruction manual for this system here


Objective lens Magnification/N.A Working Distance(mm) Immersion Medium DIC
HC PL APO 10x/0.40 2.74 Dry Yes
HC PL APO 20x/0.75 0.62 Dry Yes
HCX PL APO 40x/1.3 0.24 Oil Yes
HC PL APO 63x/1.4 0.14 Oil Yes
HCX PL APO 63x/1.2 0.22 Water Yes
HCX PL APO 100x/1.4 0.13 Oil Yes

TLL Staff who would like to receive training on this system, please sign up through the Microscopy Training Form.

External users who are interested in this system, please contact the Bioimaging Group